· Radiation damage

· Tumor size often does not correspond exactly to the degree of malignancy or ability to invade

· Detection depends on the presence of lesions

· Hysteresis

· Wholeness

· Immediate response to tumor malignancy and invasion ability

· Earlier than imaging prediction

· Poor correlation with physiopathology

· Poor sensitivity and specificity

· High sensitivity

· Good specificity

· Invasive means of examination

· Limited by the location of the lesion

· Re-sampling is limited

· Loss of scope of application after radical surgery

· Non-invasive

· Easy to sample

· Monitor at any time

· Specimen source